The cloning and mRNA expression analysis of Xiphophorus maculatus JunA and JunB proto-oncogenes (designated X-JunA and X-JunB, respectively) is described. In mammals, JunA and JunB proteins make up the activator protein-1 (AP-1) transcription factor with related Fos proteins. The deduced amino acid sequences of X-JunA and X-JunB exhibit moderate degrees of similarity when compared to their human homologues, while the regions considered functionally critical, namely, the transactivation domains, DNA-binding domain, and the leucine zipper, are highly conserved. X-JunA and X-JunB mRNA expression levels in six X. maculatus Jp 163 A tissues were assayed by real-time RT-PCR. In addition, X-JunA and X-JunB mRNA levels are compared in skin and tumor tissues derived from two distinct Xiphophorus backcross hybrid tumor models, one of which develops melanoma spontaneously, whereas the other requires induction via UVB exposure for melanoma development. X-JunB mRNA expression was higher than X-JunA expression in tissues from X. maculatus parental animals. X-JunB was also more highly expressed than X-JunA in both spontaneous and induced melanoma tissue and nonmelanotic skin tissue. However, X-JunA mRNA levels were significantly higher in the spontaneous melanomas compared to melanomas induced by UVB exposure. The authors speculate that these findings may indicate that JunA regulation is affected by regulatory differences between the two melanoma model systems.