Abstract
ATP-dependent chromatin remodelers of the CHD family play important roles during differentiation and development. Three CHD proteins, dMi-2, dChd1, and Kismet, have been described for Drosophila melanogaster. Here, we study dCHD3, a novel member of the CHD family. dCHD3 is related in sequence to dMi-2 but lacks several domains implicated in dMi-2 function. We demonstrate that dCHD3 is a nuclear protein and that expression is tightly regulated during fly development. Recombinant dCHD3 remodels mono- and polynucleosomes in an ATP-dependent manner in vitro. Its chromodomains are critical for nucleosome binding and remodeling. Unlike dMi-2, dCHD3 exists as a monomer. Nevertheless, both proteins colocalize with RNA polymerase II to actively transcribed regions on polytene chromosomes, suggesting that both remodelers participate in the process of transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases / genetics
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Adenosine Triphosphatases / metabolism
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Adenosine Triphosphate / metabolism*
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Animals
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Autoantigens / genetics
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Autoantigens / metabolism
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Chromatin Assembly and Disassembly / genetics*
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Chromosomes / genetics
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DNA / metabolism
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DNA Helicases / genetics
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DNA Helicases / metabolism*
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / embryology
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Drosophila melanogaster / genetics
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Drosophila melanogaster / growth & development
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Drosophila melanogaster / metabolism
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Gene Expression Regulation, Developmental
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Histones / metabolism
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Nucleosomes / enzymology
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Phylogeny
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Protein Binding
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Transcription, Genetic / genetics*
Substances
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Autoantigens
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Drosophila Proteins
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Histones
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Mi-2 protein, Drosophila
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Nucleosomes
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Adenosine Triphosphate
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DNA
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Adenosine Triphosphatases
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CHD3 protein, Drosophila
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DNA Helicases