Analysis of toll-like receptor-dependent production of proinflammatory cytokines in vitro by human peripheral blood mononuclears of donors and patients with primary immunodeficiency

Bull Exp Biol Med. 2007 Jul;144(1):63-5. doi: 10.1007/s10517-007-0255-z.
[Article in English, Russian]

Abstract

The production of TNF-alpha and IFN-alpha cytokines by peripheral blood mononuclears in response to stimulation by TLR2/6, TLR4, TLR5, TLR9 ligands (zymosan, LPS, flagellin, and CpG-oligodeoxynucleotide, respectively) was studied in donors and patients with common variable immunodeficiency. Individual characteristics of TNF-alpha production by mononuclears were revealed in donors. Reduced stimulated production of TNF-alpha in response to stimulation with TLR4 and TLR5 ligands in vitro was detected in patients with common variable immunodeficiency.

MeSH terms

  • Adolescent
  • Adult
  • Blood Donors*
  • Child
  • Common Variable Immunodeficiency / blood
  • Common Variable Immunodeficiency / physiopathology*
  • Female
  • Flagellin / pharmacology
  • Humans
  • Interferon-alpha / biosynthesis*
  • Leukocytes, Mononuclear
  • Lipopolysaccharides / pharmacology
  • Male
  • Oligodeoxyribonucleotides / pharmacology
  • Poly I-C / pharmacology
  • Toll-Like Receptors / drug effects
  • Toll-Like Receptors / physiology*
  • Tumor Necrosis Factor-alpha / biosynthesis*
  • Zymosan / pharmacology

Substances

  • CPG-oligonucleotide
  • Interferon-alpha
  • Lipopolysaccharides
  • Oligodeoxyribonucleotides
  • Toll-Like Receptors
  • Tumor Necrosis Factor-alpha
  • Flagellin
  • Zymosan
  • Poly I-C