The conserved extension of the Hbp autotransporter signal peptide does not determine targeting pathway specificity

Wouter S P Jong; Joen Luirink

doi:10.1016/j.bbrc.2008.01.122

The conserved extension of the Hbp autotransporter signal peptide does not determine targeting pathway specificity

Biochem Biophys Res Commun. 2008 Apr 11;368(3):522-7. doi: 10.1016/j.bbrc.2008.01.122. Epub 2008 Feb 5.

Authors

Wouter S P Jong¹, Joen Luirink

Affiliation

¹ Department of Molecular Microbiology, Institute of Molecular Cell Biology, VU University Amsterdam, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.

PMID: 18258183
DOI: 10.1016/j.bbrc.2008.01.122

Abstract

Autotransporters (ATs) of Gram-negative bacteria are often produced with an unusual signal peptide that carries a conserved N-terminal extension. Using combined in vitro and in vivo approaches we show that the extension of the AT hemoglobin protease (Hbp) does not affect targeting of Hbp via the SRP-pathway, suggesting that the extension is not involved in targeting pathway selection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Membrane / metabolism*
Conserved Sequence
Endopeptidases / metabolism*
Escherichia coli K12 / physiology*
Gene Targeting
Protein Sorting Signals / physiology*
Reproducibility of Results
Sensitivity and Specificity
Signal Recognition Particle / metabolism*

Substances

Protein Sorting Signals
Signal Recognition Particle
Endopeptidases
hemoglobin protease Hbp