Oligonucleotide microarray analysis of gene expression profiles followed by real-time reverse-transcriptase polymerase chain reaction assay in chronic active Epstein-Barr virus infection

J Infect Dis. 2008 Mar 1;197(5):663-6. doi: 10.1086/527330.

Abstract

Chronic active Epstein-Barr virus infection (CAEBV) is characterized by recurrent infectious mononucleosis-like symptoms and has high mortality and morbidity. To clarify the mechanisms of CAEBV, the gene-expression profiles of peripheral blood obtained from patients with CAEBV were investigated. Twenty genes were differentially expressed in 4 patients with CAEBV. This microarray result was verified using a real-time reverse-transcriptase polymerase chain reaction assay in a larger group of patients with CAEBV. Eventually, 3 genes were found to be significantly upregulated: guanylate binding protein 1, tumor necrosis factor-induced protein 6, and guanylate binding protein 5. These genes may be associated with the inflammatory reaction or with cell proliferation.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adolescent
  • Adult
  • Case-Control Studies
  • Cell Adhesion Molecules / metabolism
  • Child
  • Child, Preschool
  • Chronic Disease
  • GTP-Binding Proteins / metabolism
  • Gene Expression Profiling
  • Herpesvirus 4, Human / genetics*
  • Humans
  • Infectious Mononucleosis / genetics*
  • Leukocytes, Mononuclear / metabolism*
  • Leukocytes, Mononuclear / virology
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Up-Regulation

Substances

  • Cell Adhesion Molecules
  • GBP1 protein, human
  • GBP5 protein, human
  • TNFAIP6 protein, human
  • GTP-Binding Proteins