Abstract
A model for human lymphocyte ontogeny has been developed in a normal mouse. Human bone marrow, depleted of mature T and B lymphocytes, and bone marrow from mice with severe combined immunodeficiency were transplanted into lethally irradiated BALB/c mice. Human B and T cells were first detected 2 to 4 months after transplantation and persisted for at least 6 months. Most human thymocytes (30 to 50 percent of total thymocytes) were CD3+CD4+CD8+. Human immunoglobulin was detected in some chimeras, and a human antibody response to dinitrophenol could be generated after primary and secondary immunization.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies / analysis
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Antibodies, Monoclonal / immunology
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Antigens, Differentiation, T-Lymphocyte / immunology
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B-Lymphocytes / cytology*
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B-Lymphocytes / immunology
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Bone Marrow Cells*
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Bone Marrow Transplantation*
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CD3 Complex
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Cell Differentiation
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Chimera
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Hematopoietic Stem Cells / cytology
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Hemocyanins / immunology
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Humans
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Immunization
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Immunoglobulins / analysis
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Immunologic Deficiency Syndromes
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Immunophenotyping
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Mice
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Mice, Inbred BALB C
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Receptors, Antigen, T-Cell / immunology
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T-Lymphocytes / cytology*
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T-Lymphocytes / immunology
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Transplantation, Heterologous*
Substances
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2,4-dinitrophenyl keyhole limpet hemocyanin
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Antibodies
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Antibodies, Monoclonal
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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Immunoglobulins
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Receptors, Antigen, T-Cell
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Hemocyanins