Abstract
We isolated three class I and three class II KNOX genes in Medicago truncatula. The predicted amino acid sequences suggested a possible orthology to the Arabidopsis homeodomain proteins STM, KNAT1/BP, KNAT3 and KNAT7 that was confirmed by phylogenetic and conserved structural domain analyses. Moreover, the STM-like MtKNOX1 and MtKNOX6 proteins were shown to retain the capability to interact with the Arabidopsis BELL protein partners of STM and KNAT1/BP. Amino acid residues that characterize the different classes of KNOX proteins were identified. Gene expression studies revealed organ-specificity, possible cytokinin-dependent transcriptional activation of two MtKNOXs and expression of one STM-like and a BP/KNAT1-like MtKNOX in roots. Interestingly, mRNA localization studies carried out on class I MtKNOX genes revealed important differences with previously characterised legume KNOXs. M. truncatula transcripts were not down-regulated in leaf primordia and early stages of leaf development, features shared with the more distant compound-leaved species Solanum lycopersicum.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cloning, Molecular
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Cytokinins / pharmacology
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Gene Expression Regulation, Plant / drug effects
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Gibberellins / pharmacology
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Homeodomain Proteins / chemistry
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Homeodomain Proteins / genetics*
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Homeodomain Proteins / metabolism
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Medicago truncatula / drug effects
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Medicago truncatula / genetics*
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Medicago truncatula / metabolism
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Meristem / metabolism
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Molecular Sequence Data
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Multigene Family
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Phylogeny
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Plant Growth Regulators / pharmacology
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Plant Proteins / chemistry
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Plant Proteins / genetics*
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Plant Proteins / metabolism
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Plant Roots / genetics
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Plant Roots / metabolism
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Plant Shoots / genetics
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Plant Shoots / metabolism
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RNA, Messenger / metabolism
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Sequence Alignment
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Transcription Factors / chemistry
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Transcription Factors / genetics*
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Transcription Factors / metabolism
Substances
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Cytokinins
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Gibberellins
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Homeodomain Proteins
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Plant Growth Regulators
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Plant Proteins
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RNA, Messenger
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Transcription Factors
Associated data
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GENBANK/AB033478
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GENBANK/AB033479
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GENBANK/AB033480
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GENBANK/AB033481
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GENBANK/AB033482
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GENBANK/AB033483
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GENBANK/EF128056
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GENBANK/EF128057
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GENBANK/EF128058
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GENBANK/EF128059
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GENBANK/EF128060
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GENBANK/EF128061