Abstract
A new pyranonaphthoquinone antibiotic, griseusin D (1) was isolated from the cultural fluid of the alkaphilic Nocardiopsis sp. The structure was determined as 5'-one-4-hydroxy-12-methoxygriseusin by spectroscopic methods, comparison with reported data and single-crystal X-ray analysis. 1 displayed strong cytotoxicity against human leukemia cells (HL60) and modest cytotoxicity against human lung adenocarcinoma cell lines (AGZY) with IC(50) values of 0.23 and 19.6 microg/ml, respectively. It also exhibited weak antifungal activity against Alternaria alternate with MIC of 140 microg/ml.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / metabolism*
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Alternaria / drug effects
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Antibiotics, Antineoplastic / chemistry*
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Antibiotics, Antineoplastic / isolation & purification
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Antibiotics, Antineoplastic / pharmacology*
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Antifungal Agents / chemistry*
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Antifungal Agents / isolation & purification
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Antifungal Agents / pharmacology*
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Cell Line, Tumor
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Crystallography, X-Ray
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Culture Media / chemistry
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Humans
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Inhibitory Concentration 50
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Microbial Sensitivity Tests
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Molecular Structure
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Naphthoquinones / chemistry
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Naphthoquinones / isolation & purification
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Naphthoquinones / pharmacology
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Spectrum Analysis
Substances
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Antibiotics, Antineoplastic
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Antifungal Agents
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Culture Media
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Naphthoquinones
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griseusin