A recently developed Latex agglutination method known as "KATEX" for detecting leishmanial antigen in urine of Kala-azar patients was evaluated on 97 Kala-azar cases and 35 controls in the department of Microbiology, Mymensingh Medical College during the period from March' 2004 to February' 2005. The method yielded sensitivity as 100% and 82.8% in 33 confirmed and 64 ICT positive cases respectively. Since 8.6% controls showed antigen positive results, so specificity of KATEX was calculated as 91.4%. KATEX methods for antigen detection in urine should be used as an early immuno-diagnostic test as it has yielded high sensitivity. But interpretation of a positive test must be made cautiously having correlation with clinical findings as because it becomes false positive in Kala-azar free person. Further elucidation of KATEX method including larger population from community giving particular emphasis on its prognostic use was strongly recommended.