Abstract
A reproducible and rapid procedure for isolation of cloned cDNA insert from a lambda gt11 cDNA library is described. The procedure relies on the polymerase chain reaction method using forward and reverse primers for lambda gt11, followed by isolation of the cloned cDNA insert by a rapid technique. The procedure should also be applicable to isolation of cDNA inserts cloned in other vectors such as lambda gt10.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bacteriophage lambda / genetics
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Base Sequence
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Cloning, Molecular*
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DNA / isolation & purification*
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DNA Probes
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Deoxyribonuclease EcoRI
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Drosophila melanogaster / genetics
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Molecular Sequence Data
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Polymerase Chain Reaction*
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Protein Kinases / genetics
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Protein Serine-Threonine Kinases
Substances
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DNA Probes
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DNA
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Protein Kinases
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Protein Serine-Threonine Kinases
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Deoxyribonuclease EcoRI