Immunoelectron microscopic characterization of a subpopulation of freshly isolated epidermal Langerhans cells that reacts with anti-CD23 monoclonal antibody

C Torresani; G C Manara; C Ferrari; G De Panfilis

doi:10.1111/j.1365-2133.1991.tb04945.x

Immunoelectron microscopic characterization of a subpopulation of freshly isolated epidermal Langerhans cells that reacts with anti-CD23 monoclonal antibody

Br J Dermatol. 1991 Jun;124(6):533-7. doi: 10.1111/j.1365-2133.1991.tb04945.x.

Authors

C Torresani¹, G C Manara, C Ferrari, G De Panfilis

Affiliation

¹ Department of Dermatology, Parma University, Italy.

PMID: 1829625
DOI: 10.1111/j.1365-2133.1991.tb04945.x

Abstract

Large subsets of leucocytes were recently shown to express the low affinity receptor for the Fc portion of IgE. Because Langerhans cells (LC) are epidermal leucocytes, we investigated whether LC of normal human subjects might express this receptor. Whereas conventional immunofluorescence on epidermal sheets gave negative results, highly sensitive immunoelectron microscopy revealed that a subset (about one-third) of freshly isolated LC express the CD23 molecule.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antigens, CD / immunology*
Antigens, Differentiation, B-Lymphocyte / analysis*
Fluorescent Antibody Technique
Humans
Immunoglobulin E / immunology*
Langerhans Cells / immunology*
Langerhans Cells / ultrastructure
Microscopy, Immunoelectron*
Middle Aged
Receptors, Fc / analysis*
Receptors, IgE

Substances

Antigens, CD
Antigens, Differentiation, B-Lymphocyte
Receptors, Fc
Receptors, IgE
Immunoglobulin E