Objectives: To characterize the prevalence of plasmid-mediated quinolone resistance determinants qnr and aac(6')-Ib-cr in extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae.
Methods: qnrA, qnrB, qnrS, aac(6')-Ib-cr and ESBL-encoding genes were detected by PCR. MICs of 10 antimicrobial agents were determined by Etest. PFGE was used to investigate the clonality of qnr- and aac(6')-Ib-cr-producing isolates. Conjugation and Southern hybridizations were used to confirm whether qnr, aac(6')-Ib-cr or ESBL-encoding genes were located on plasmids.
Results: Twenty-nine (8.0%) of 362 isolates were positive for qnr genes, and the qnrA-, qnrB- and qnrS-type genes were detected alone or in combination in 13 (3.6%), 8 (2.2%) and 9 (2.5%), respectively. Sixty-two (17.1%) isolates were positive for aac(6')-Ib, of which 36 (9.9% of all) had the -cr variant. Conjugation and Southern hybridization revealed that qnrA, aac(6')-Ib-cr and ESBL-encoding genes were always located on the same plasmids.
Conclusions: qnr and aac(6')-Ib-cr genes were detected in 8.0% and 9.9% of ESBL-producing E. coli and K. pneumoniae, respectively. The plasmids carrying the qnr gene could be transferred by conjugation together with ESBL-encoding genes and aac(6')-Ib-cr.