Abstract
In contrast to methicillin-resistant Staphylococcus aureus, little is known of the distribution of spa types among methicillin-susceptible S. aureus (MSSA). We have analyzed 101 nonrepetitive invasive MSSA isolates from infected patients, consecutively isolated during 14 months between 2006 and 2007 at University Hospital Basel. They were genetically characterized according to S. aureus protein A (spa) types and important virulence-associated genes. Sixty-five different spa types corresponding to nine different spa clonal complexes were observed. Analysis of different virulence genes showed a frequency of 17% for toxic-shock syndrome toxin and 5% for exfoliative toxin D. In conclusion, spa typing revealed a great genetic diversity without predominant spa type, not providing evidence for clonal spreading.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Anti-Bacterial Agents / pharmacology*
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Bacterial Toxins / genetics
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Bacterial Typing Techniques
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Cluster Analysis
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Cross Infection / epidemiology
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Cross Infection / microbiology*
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DNA, Bacterial / genetics
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Enterotoxins / genetics
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Exfoliatins / genetics
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Female
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Genetic Variation
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Genotype
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Hospitals, University
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Humans
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Male
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Methicillin / pharmacology*
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Middle Aged
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Molecular Epidemiology
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Polymerase Chain Reaction
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Staphylococcal Infections / epidemiology
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Staphylococcal Infections / microbiology*
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Staphylococcal Protein A / genetics
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Staphylococcus aureus / classification*
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Staphylococcus aureus / drug effects*
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Staphylococcus aureus / genetics
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Staphylococcus aureus / isolation & purification
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Superantigens / genetics
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Switzerland / epidemiology
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Virulence Factors / genetics
Substances
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Anti-Bacterial Agents
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Bacterial Toxins
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DNA, Bacterial
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Enterotoxins
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Exfoliatins
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Staphylococcal Protein A
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Superantigens
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Virulence Factors
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enterotoxin F, Staphylococcal
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Methicillin