Comparison between the effects of the microsomal Ca(2+)-translocase inhibitors thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone on cellular calcium fluxes

Biochem J. 1991 Jul 15;277 ( Pt 2)(Pt 2):553-6. doi: 10.1042/bj2770553.

Abstract

The effects of two inhibitors of the microsomal Ca(2+)-ATPase, thapsigargin and 2,5-di-(t-butyl)-1,4-benzohydroquinone, were compared in hepatocytes and in a T-cell line (JURKAT). Both compounds mobilized the same intracellular Ca2+ pool, which contained the Ins(1,4,5)P3-sensitive store, in hepatocytes and in JURKAT cells. The mobilization of the internal Ca2+ store with either compound activated Mn2+ entry in JURKAT cells, but not in hepatocytes. This suggests different properties of the bivalent-cation entry pathway between these cell types.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / pharmacology
  • Calcium / metabolism*
  • Calcium-Transporting ATPases / antagonists & inhibitors*
  • Cell Line
  • Cells, Cultured
  • Cytosol / metabolism
  • Fura-2
  • Humans
  • Hydroquinones / pharmacology*
  • Kinetics
  • Leukemia, T-Cell
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Microsomes, Liver / enzymology
  • Rats
  • Rats, Inbred Strains
  • Signal Transduction / drug effects
  • Terpenes / pharmacology*
  • Thapsigargin
  • Vasopressins / pharmacology

Substances

  • Antioxidants
  • Hydroquinones
  • Terpenes
  • Vasopressins
  • 2,5-di-tert-butylhydroquinone
  • Thapsigargin
  • Calcium-Transporting ATPases
  • Calcium
  • Fura-2