Expansion of circulating NKG2D+ effector memory T-cells and expression of NKG2D-ligand MIC in granulomaous lesions in Wegener's granulomatosis

Clin Immunol. 2008 May;127(2):144-50. doi: 10.1016/j.clim.2007.12.004. Epub 2008 Mar 4.

Abstract

Expansion of circulating CD28- T-cells reminiscent of effector memory T-cells (T(EM)) has been reported in Wegener's granulomatosis (WG) recently. To investigate the role of T(EM) in WG, we analyzed the expression of the activating NK-receptor NKG2D and its ligand MIC on circulating T(EM) and in granulomatous lesions, respectively. NKG2D was anomalously expressed and preferentially detected on circulating CD4+CD28- T(EM) in WG. Compared to healthy controls, T(EM) display a more activated phenotype potentially favoring unbalanced proinflammatory responses in WG. Cluster-like formations of "Wegener's autoantigen" PR3 were surrounded by NKG2D+ and NKG2D-ligand MIC+ cells in WG-granulomata, but not in disease controls. Further, IL-15 - known to drive T(EM) differentiation and proliferation--was also expressed in WG-granulomata. Thus, through acquisition of NK-like "innate" properties, IL-15 stimulated NKG2D+ T(EM) could interact with MIC+ cells within WG-granulomata, thereby sustaining inflammation and autoimmunity and promoting self-perpetuating pathology in WG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biopsy
  • CD28 Antigens / immunology
  • CD4-Positive T-Lymphocytes / immunology*
  • Flow Cytometry
  • GPI-Linked Proteins
  • Granulomatosis with Polyangiitis / blood
  • Granulomatosis with Polyangiitis / immunology*
  • Humans
  • Immunohistochemistry
  • Immunologic Memory / immunology*
  • Intercellular Signaling Peptides and Proteins / biosynthesis
  • Intercellular Signaling Peptides and Proteins / immunology*
  • Interleukin-15 / biosynthesis
  • Interleukin-15 / immunology
  • Myeloblastin / immunology
  • NK Cell Lectin-Like Receptor Subfamily K
  • Receptors, Immunologic / biosynthesis
  • Receptors, Immunologic / blood
  • Receptors, Immunologic / immunology*
  • Receptors, Natural Killer Cell

Substances

  • CD28 Antigens
  • GPI-Linked Proteins
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-15
  • KLRK1 protein, human
  • NK Cell Lectin-Like Receptor Subfamily K
  • Receptors, Immunologic
  • Receptors, Natural Killer Cell
  • ULBP2 protein, human
  • Myeloblastin