Abstract
Eight female Peromyscus californicus were infected with 10(2) or 10(4) Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 10(4) tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Antibodies, Protozoan / biosynthesis*
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Brain / parasitology
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DNA, Protozoan / analysis
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Disease Susceptibility
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Female
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Fluorescent Antibody Technique, Indirect
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Heart / parasitology
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Immunohistochemistry
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Otters
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Peromyscus / parasitology*
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Polymerase Chain Reaction
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Random Allocation
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Rodent Diseases / immunology*
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Rodent Diseases / parasitology
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Rodent Diseases / transmission
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Spleen / parasitology
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Toxoplasma / genetics
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Toxoplasma / immunology*
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Toxoplasma / isolation & purification
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Toxoplasmosis, Animal / immunology*
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Toxoplasmosis, Animal / transmission
Substances
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Antibodies, Protozoan
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DNA, Protozoan