Abstract
CD97 is broadly expressed on hematopoietic cells and is involved in neutrophil migration. Since neutrophils are key regulators in HSC/HPC mobilization, we studied a possible role for CD97 in interleukin-8 and granulocyte-colony stimulating factor-induced HSC/HPC mobilization. Mobilization was absent in mice receiving CD97 mAb followed by interleukin-8, while granulocyte-colony stimulating factor-induced mobilization remained unaltered following anti-CD97 administration. Furthermore, combined administration of CD97 mAb and IL-8 induced a significant reduction in the neutrophilic compartment. We hypothesize that the absence of interleukin-8-induced HSC/HPC mobilization after CD97 mAb administration is due to its effect on neutrophil function.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Antibodies, Monoclonal / administration & dosage
-
Antibodies, Monoclonal / pharmacology*
-
Chemotaxis, Leukocyte / drug effects*
-
Colony-Forming Units Assay
-
Drug Administration Schedule
-
Granulocyte Colony-Stimulating Factor / administration & dosage
-
Granulocyte Colony-Stimulating Factor / pharmacology*
-
Hematopoietic Stem Cell Mobilization / methods*
-
Interleukin-8 / administration & dosage
-
Interleukin-8 / pharmacology*
-
Leukocyte Count
-
Male
-
Membrane Glycoproteins / antagonists & inhibitors
-
Membrane Glycoproteins / immunology
-
Membrane Glycoproteins / physiology*
-
Mice
-
Mice, Inbred BALB C
-
Neutrophils / physiology
-
Receptors, G-Protein-Coupled
-
Recombinant Fusion Proteins / pharmacology
Substances
-
Adgre5 protein, mouse
-
Antibodies, Monoclonal
-
Interleukin-8
-
Membrane Glycoproteins
-
Receptors, G-Protein-Coupled
-
Recombinant Fusion Proteins
-
Granulocyte Colony-Stimulating Factor