Abstract
We have cloned the nodFE operon from Sinorhizobium sp. strain MUS10. MUS10 NodF shows sequence homology to acyl carrier protein and enables an S. meliloti nodF mutant to effectively nodulate alfalfa. Our results demonstrate the occurrence of nodFE in a symbiont that nodulates a legume host not belonging to the galegoid group.
MeSH terms
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Acyl Carrier Protein / genetics
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Amino Acid Sequence
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Bacterial Proteins / genetics*
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Bacterial Proteins / physiology
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Blotting, Southern
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Cloning, Molecular
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Gene Deletion
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Medicago sativa / microbiology
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Molecular Sequence Data
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Sequence Alignment
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
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Sesbania / microbiology
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Sinorhizobium / genetics*
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Sinorhizobium / physiology
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Symbiosis
Substances
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Acyl Carrier Protein
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Bacterial Proteins
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DNA, Bacterial