Abstract
Chromosomal region 11q23 participates in a number of reciprocal translocations with specific regions of chromosomes 4, 9, 19, and others. These translocations are associated with acute lymphocytic leukemia and acute myelomonocytic, monocytic, and myelogenous leukemia. From a yeast artificial chromosome containing human DNA derived from 11q23 we cloned a DNA fragment which can be used as a probe to detect rearrangements in leukemic cells from the majority of patients with the t(4;11), t(9;11), and t(11;19) translocations. The breakpoints cluster in a small DNA region of less than 5.8 kilobases.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Antigens, Differentiation, T-Lymphocyte / genetics
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Blotting, Northern
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Blotting, Southern
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CD3 Complex
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Chromosome Mapping
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Chromosomes, Human, Pair 11*
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Chromosomes, Human, Pair 19
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Chromosomes, Human, Pair 4
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Chromosomes, Human, Pair 9
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Cloning, Molecular
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DNA, Neoplasm / genetics
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Humans
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Precursor Cell Lymphoblastic Leukemia-Lymphoma / genetics*
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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Receptors, Antigen, T-Cell / genetics
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Restriction Mapping
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Translocation, Genetic / genetics*
Substances
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Antigens, Differentiation, T-Lymphocyte
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CD3 Complex
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DNA, Neoplasm
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RNA, Messenger
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RNA, Neoplasm
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Receptors, Antigen, T-Cell