Abstract
The formation of clathrin-coated endocytic vesicles is driven by a complex and highly dynamic molecular machinery. In this issue, Idrissi et al. (Idrissi, F.-Z., H. Grötsch, I.M. Fernández-Golbano, C. Presciatto-Baschong, H. Riezman, and M.-I. Geli. 2008. J. Cell Biol. 180:1219-1232) reveal some of the secrets of this machinery by analyzing the localizations of nine endocytic proteins during vesicle budding in yeast using quantitative immunoelectron microscopy.
MeSH terms
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Cell Membrane / metabolism
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Cell Membrane / ultrastructure
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Clathrin / metabolism
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Clathrin-Coated Vesicles / metabolism*
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Clathrin-Coated Vesicles / ultrastructure
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Endocytosis / physiology*
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Gene Expression Regulation, Fungal / genetics
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Microscopy, Immunoelectron / methods
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Protein Transport
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae / ultrastructure
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism
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Transport Vesicles / metabolism*
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Transport Vesicles / ultrastructure
Substances
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Clathrin
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Saccharomyces cerevisiae Proteins