Abstract
Primary cilia are sensory structures on the cell surface whose formation requires tight integration of microtubules and polarized membrane trafficking events. Rabs are GTP-binding proteins of the Ras superfamily that control directed membrane trafficking by regulating membrane-membrane and membrane-cytoskeleton interactions. This chapter describes cell biological and biochemical methods for the analysis of Rab function and Rab GTPase-activating proteins during primary cilia formation.
MeSH terms
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Cell Cycle Proteins
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Cell Line
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Cilia / metabolism*
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Cloning, Molecular
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GTPase-Activating Proteins / analysis*
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Heat-Shock Proteins / analysis
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Heat-Shock Proteins / physiology
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Humans
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Microtubule-Associated Proteins / analysis
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Nuclear Proteins / isolation & purification
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rab GTP-Binding Proteins / analysis*
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rab GTP-Binding Proteins / physiology
Substances
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Cell Cycle Proteins
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EVI5 protein, human
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GTPase-Activating Proteins
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Heat-Shock Proteins
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Microtubule-Associated Proteins
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Nuclear Proteins
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ODF2 protein, human
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RABGAP1 protein, human
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RAB8A protein, human
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rab GTP-Binding Proteins