Radiofungicidal effects of external gamma radiation and antibody-targeted beta and alpha radiation on Cryptococcus neoformans

Antimicrob Agents Chemother. 2008 Jun;52(6):2232-5. doi: 10.1128/AAC.01245-07. Epub 2008 Mar 31.

Abstract

We evaluated the clonogenic survival, membrane permeability, metabolic activity (XTT reduction), and apoptosis (FLICA binding) of Cryptococcus neoformans cells subjected to gamma rays from an external source, and beta and alpha particles delivered to fungal cells by capsule-specific antibody. We found that gamma, beta, and alpha radiation affected cells through different pathways.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alpha Particles*
  • Animals
  • Antibodies, Monoclonal
  • Apoptosis / radiation effects
  • Beta Particles*
  • Bismuth
  • Cell Membrane Permeability / radiation effects*
  • Colony Count, Microbial
  • Cryptococcus neoformans / growth & development
  • Cryptococcus neoformans / metabolism*
  • Cryptococcus neoformans / radiation effects*
  • Gamma Rays*
  • Humans
  • Radioisotopes
  • Rhenium
  • Tetrazolium Salts / metabolism

Substances

  • Antibodies, Monoclonal
  • Radioisotopes
  • Tetrazolium Salts
  • 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide
  • Rhenium
  • Bismuth