Abstract
Two new bis-coumarin glycosides were isolated from the stem barks of Daphne giraldii NITSCHE. Their structures were elucidated as 6-O-alpha-L-rhamnnopyranosyl daphnogirin (1), and 6-O-beta-D-apiofuranosyl daphnogirin (2), on the basis of detailed spectroscopic analysis. Compounds 1 and 2 were tested inhibitory effects against production of nitric oxide (NO) in RAW264.7 cells, and cytotoxicity of human tumor cell lines A549, LOVO, QGY-7703, 6T-CEM. The results showed that compounds 1 and 2 neither reduced production of NO, nor inhibited human tumor cell lines.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry*
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Antineoplastic Agents, Phytogenic / pharmacology*
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Carbohydrate Conformation
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Cell Line, Tumor
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Coumarins / chemistry*
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Coumarins / pharmacology*
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Daphne / chemistry*
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Drug Screening Assays, Antitumor
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / pharmacology*
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Glycosides / chemistry*
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Glycosides / pharmacology*
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Humans
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Hydrolysis
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Macrophages / drug effects
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Macrophages / enzymology
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Magnetic Resonance Spectroscopy
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Nitric Oxide Synthase / antagonists & inhibitors*
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Plant Bark / chemistry
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Tetrazolium Salts
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Thiazoles
Substances
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6-O-alpha-L-rhamnnopyranosyl daphnogirin
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6-O-beta-D-apiofuranosyl daphnogirin
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Antineoplastic Agents, Phytogenic
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Coumarins
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Enzyme Inhibitors
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Glycosides
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Tetrazolium Salts
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Thiazoles
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Nitric Oxide Synthase
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thiazolyl blue