Abstract
Subtractive hybridization was carried out to identify differences between the sequenced genome of Bordetella pertussis Tohama I and those of two recently collected isolates. We identified genetic regions specific to recent isolates, old isolates, and isolates of B. parapertussis and B. bronchiseptica species. We conclude that Tohama I strain is not representative of the B. pertussis species.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bordetella pertussis / classification*
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Bordetella pertussis / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Electrophoresis, Gel, Pulsed-Field
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Genome, Bacterial*
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Humans
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Nucleic Acid Hybridization / methods*
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Reference Standards
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Sequence Analysis, DNA*
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Species Specificity
Substances
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Deoxyribonucleases, Type II Site-Specific
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GATC-specific type II deoxyribonucleases
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GTAC-specific type II deoxyribonucleases