Grafting BSA onto poly[(L-lactide)-co-carbonate] microspheres by click chemistry

Macromol Biosci. 2008 Jul 7;8(7):638-44. doi: 10.1002/mabi.200700306.

Abstract

Model protein bovine serum albumin (BSA) was covalently grafted onto poly[(L-lactide)-co-carbonate] microsphere surfaces by "click chemistry." The grafting was confirmed by confocal laser scanning microscopy and X-ray photoelectron spectroscopy. The maximum amount of surface-grafted BSA was 45 mg x g(-1). The secondary structure of the grafted BSA was analyzed by FTIR and the results demonstrated that the grafting did not affect protein structure. This strategy can also be used on microspheres prepared from poly(L-lactide)/poly[(L-lactide)-co-carbonate] blend materials.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Microspheres*
  • Polyesters / chemical synthesis
  • Polyesters / chemistry*
  • Protein Structure, Secondary
  • Serum Albumin, Bovine / chemistry*
  • Spectroscopy, Fourier Transform Infrared

Substances

  • Polyesters
  • Serum Albumin, Bovine