The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome

Kambiz Gilany; Roos Van Elzen; Kim Mous; Edmond Coen; Walter Van Dongen; Stefaan Vandamme; Kris Gevaert; Evy Timmerman; Joël Vandekerckhove; Sylvia Dewilde; Xaveer Van Ostade; Luc Moens

doi:10.1016/j.bbapap.2008.03.003

The proteome of the human neuroblastoma cell line SH-SY5Y: an enlarged proteome

Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):983-5. doi: 10.1016/j.bbapap.2008.03.003. Epub 2008 Mar 19.

Authors

Kambiz Gilany¹, Roos Van Elzen, Kim Mous, Edmond Coen, Walter Van Dongen, Stefaan Vandamme, Kris Gevaert, Evy Timmerman, Joël Vandekerckhove, Sylvia Dewilde, Xaveer Van Ostade, Luc Moens

Affiliation

¹ Department of Biomedical Sciences, Protein Chemistery, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.

PMID: 18402783
DOI: 10.1016/j.bbapap.2008.03.003

Abstract

The human neuroblastoma cell line SH-SY5Y (ATCC: CRL-2266) is widely used as a neural cellular model system. The hitherto existing proteome data (115 proteins) are here extended. A total of 1103 unique proteins of this cell line were identified using 2D-LC combined with MALDI-TOF/TOF-MS, SDS-PAGE with nano-LC-MS/MS, N-terminal COFRADIC analysis with nano-LC-MS/MS and 2D-PAGE with MALDI-TOF/TOF-MS peptide mass fingerprinting. The obtained proteome profile of this cell line is discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Electrophoresis, Polyacrylamide Gel
Humans
Neoplasm Proteins / metabolism*
Neuroblastoma / metabolism*
Neuroblastoma / pathology
Proteome*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

Neoplasm Proteins
Proteome