Scintillation proximity assay

Curr Protoc Neurosci. 2005 Feb:Chapter 7:Unit 7.15. doi: 10.1002/0471142301.ns0715s30.

Abstract

Scintillation proximity assay technologies provide a rapid non-separation method to measure common biological interactions using radioactively tagged molecules. This unit identifies potential uses of the technology for the measurement of receptor-ligand binding, cAMP accumulation, GTP binding to heterotrimeric G proteins, protease activity and cellular uptake.

MeSH terms

  • Animals
  • Biological Assay / methods*
  • Cyclic AMP / metabolism
  • Dose-Response Relationship, Drug
  • GTP-Binding Proteins / metabolism
  • Protein Binding / drug effects
  • Radioligand Assay*
  • Receptors, Serotonin / metabolism
  • Scintillation Counting / methods*
  • Serotonin / pharmacokinetics
  • Serotonin Agents / pharmacology
  • Time Factors

Substances

  • Receptors, Serotonin
  • Serotonin Agents
  • serotonin 1E receptor
  • Serotonin
  • Cyclic AMP
  • GTP-Binding Proteins