Light and dark-induced variations of the ERG c-wave voltage were recorded in control chickens and after intravitreal injection of Na aspartate, a treatment whose main effect is to functionally disconnect the pigment epithelium-photoreceptor complex from second order neurons. After aspartate, the fast light rise which characterizes this preparation is no longer observed; it is substituted for by a potential variation of much slower time course and of lower magnitude. The data totally confirm previous findings obtained through an indirect EOG technique and suggest the participation of inner retinal layers in the generation of the light peak in the chicken eye.