Optimization of a laboratory-developed test utilizing roche analyte-specific reagents for detection of Staphylococcus aureus, methicillin-resistant S. aureus, and vancomycin-resistant Enterococcus species

J Clin Microbiol. 2008 Jul;46(7):2377-80. doi: 10.1128/JCM.00230-08. Epub 2008 Apr 30.

Abstract

Nasal and perianal swab specimens were tested for detection of Staphylococcus aureus and vancomycin-resistant Enterococcus species (VRE) using a laboratory-developed real-time PCR test and microbiological cultures. The real-time PCR and culture results for S. aureus were similar. PCR had adequate sensitivity, but culture was more specific for the detection of VRE.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anal Canal / microbiology
  • Bacteriological Techniques / methods*
  • Enterococcus / drug effects
  • Enterococcus / genetics
  • Enterococcus / growth & development
  • Enterococcus / isolation & purification*
  • Humans
  • Methicillin Resistance*
  • Nose / microbiology
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Staphylococcus aureus / drug effects
  • Staphylococcus aureus / genetics
  • Staphylococcus aureus / growth & development
  • Staphylococcus aureus / isolation & purification*
  • Vancomycin Resistance*