[Serologic diagnostics of Legionella infection]

Zh Mikrobiol Epidemiol Immunobiol. 2008 Mar-Apr:(2):46-50.
[Article in Russian]

Abstract

Technical approaches to construction of preparations for serologic diagnostics of Legionella infection were presented in the article; antigenic- and immunoglobulin-based diagnostic kits with known characteristics were developed. Immunogenic properties of protein and lypopolysaccharide antigens, which have diagnostic value, were studied; similarity of protein antigens from 7 serogroups of L. pneumophila was demonstrated. Soluble antigen with known composition was obtained and used for the development of antigen-based polymeric kit for diagnostics of Legionella infection. On the basis of hyperimmune sera, immunoglobulin-based polymeric diagnostic kit and array of coagglutinating diagnostic kits for the mentioned 7 serogroups were developed. Antigen-based polymeric diagnostic kit was recommended for licensure.

Publication types

  • English Abstract

MeSH terms

  • Agglutination
  • Animals
  • Antibodies, Bacterial / immunology*
  • Antibody Specificity
  • Antigens, Bacterial / analysis
  • Antigens, Bacterial / immunology
  • Bacterial Outer Membrane Proteins / immunology
  • Bacteriological Techniques / methods*
  • Humans
  • Immune Sera
  • Immunoenzyme Techniques
  • Immunoglobulins
  • Legionella / immunology*
  • Legionellosis / diagnosis*
  • Legionellosis / microbiology
  • Legionellosis / urine
  • Lipopolysaccharides / analysis
  • Lipopolysaccharides / immunology
  • Microspheres
  • Polymers
  • Rabbits
  • Reagent Kits, Diagnostic / microbiology
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial
  • Bacterial Outer Membrane Proteins
  • Immune Sera
  • Immunoglobulins
  • Lipopolysaccharides
  • Polymers
  • Reagent Kits, Diagnostic