Protein disorder is positively correlated with gene expression in Escherichia coli

J Proteome Res. 2008 Jun;7(6):2234-45. doi: 10.1021/pr800055r. Epub 2008 May 9.

Abstract

We considered, on a global scale, the relationship between the predicted fraction of protein disorder and the RNA and protein expression in Escherichia coli. Fraction of protein disorder correlated positively with both measured RNA expression levels of E. coli genes in three different growth media and with predicted abundance levels of E. coli proteins. Though weak, the correlation was highly significant. Correlation of protein disorder with RNA expression did not depend on the growth rate of E. coli cultures and was not caused by a small subset of genes showing exceptionally high concordance in their disorder and expression levels. Global analysis was complemented by detailed consideration of several groups of proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetyl-CoA Carboxylase / chemistry
  • Acetyl-CoA Carboxylase / genetics
  • Acetyl-CoA Carboxylase / metabolism
  • Acyl Carrier Protein / chemistry
  • Acyl Carrier Protein / genetics
  • Acyl Carrier Protein / metabolism
  • Acyltransferases / chemistry
  • Acyltransferases / genetics
  • Acyltransferases / metabolism
  • Apoproteins / chemistry
  • Apoproteins / genetics
  • Apoproteins / metabolism
  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Outer Membrane Proteins / metabolism
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • Bacterial Proton-Translocating ATPases / chemistry
  • Bacterial Proton-Translocating ATPases / genetics
  • Bacterial Proton-Translocating ATPases / metabolism
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Chaperonin 10 / chemistry
  • Chaperonin 10 / genetics
  • Chaperonin 10 / metabolism
  • Culture Media / pharmacology
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • DNA-Directed RNA Polymerases / chemistry
  • DNA-Directed RNA Polymerases / genetics
  • DNA-Directed RNA Polymerases / metabolism
  • Dihydrolipoyllysine-Residue Acetyltransferase / chemistry
  • Dihydrolipoyllysine-Residue Acetyltransferase / genetics
  • Dihydrolipoyllysine-Residue Acetyltransferase / metabolism
  • Escherichia coli / drug effects
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / chemistry
  • Escherichia coli Proteins / genetics*
  • Escherichia coli Proteins / metabolism
  • Fatty Acid Synthase, Type II
  • Gene Expression Profiling*
  • Gene Expression Regulation, Bacterial*
  • Glycine Decarboxylase Complex H-Protein / chemistry
  • Glycine Decarboxylase Complex H-Protein / genetics
  • Glycine Decarboxylase Complex H-Protein / metabolism
  • Lipoproteins / chemistry
  • Lipoproteins / genetics
  • Lipoproteins / metabolism
  • Molecular Chaperones / chemistry
  • Molecular Chaperones / genetics
  • Molecular Chaperones / metabolism
  • Oligonucleotide Array Sequence Analysis
  • Peptide Elongation Factors / chemistry
  • Peptide Elongation Factors / genetics
  • Peptide Elongation Factors / metabolism
  • Peptidoglycan / chemistry
  • Peptidoglycan / genetics
  • Peptidoglycan / metabolism
  • Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics
  • Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism
  • Prokaryotic Initiation Factor-1 / chemistry
  • Prokaryotic Initiation Factor-1 / genetics
  • Prokaryotic Initiation Factor-1 / metabolism
  • Protein Conformation
  • Ribosomal Proteins / chemistry
  • Ribosomal Proteins / genetics
  • Ribosomal Proteins / metabolism
  • Transcription Factors / chemistry
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcriptional Elongation Factors

Substances

  • Acyl Carrier Protein
  • Apoproteins
  • Bacterial Outer Membrane Proteins
  • Bacterial Proteins
  • Carrier Proteins
  • Chaperonin 10
  • Culture Media
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • ExcC protein, E coli
  • Glycine Decarboxylase Complex H-Protein
  • H-NS protein, bacteria
  • Lipoproteins
  • Molecular Chaperones
  • NusG protein, E coli
  • Peptide Elongation Factors
  • Peptidoglycan
  • Prokaryotic Initiation Factor-1
  • Ribosomal Proteins
  • Transcription Factors
  • Transcriptional Elongation Factors
  • acpP protein, E coli
  • nusA protein, E coli
  • Skp protein, E coli
  • Acyltransferases
  • Dihydrolipoyllysine-Residue Acetyltransferase
  • dihydrolipoamide succinyltransferase
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • RNA polymerase omega subunit
  • DNA-Directed RNA Polymerases
  • Bacterial Proton-Translocating ATPases
  • Fatty Acid Synthase, Type II
  • Acetyl-CoA Carboxylase
  • biotin carboxyl carrier protein