Abstract
We show that transcription factor Krüppel-like factor 5 (KLF5), which is important in cardiovascular remodeling, interacts with retinoic acid receptor-alpha (RARalpha) to regulate downstream gene expression. Here, we investigated whether acyclic retinoid (ACR) regulates KLF5 and inhibits vascular remodeling. Co-immunoprecipitation and pull-down binding assay showed that ACR attenuates functional interaction of KLF5 and RARalpha. ACR affects KLF5 functions by regulating transactivation of platelet-derived growth factor A (PDGF-A) chain. ACR may be a new vascular therapy to target KLF5 in cardiovascular pathology.
MeSH terms
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Animals
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Antineoplastic Agents / pharmacology*
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Cattle
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Cells, Cultured
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Immunoprecipitation
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Kruppel-Like Transcription Factors / antagonists & inhibitors*
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Kruppel-Like Transcription Factors / metabolism
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Male
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Mice
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Mice, Inbred C57BL
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Neovascularization, Pathologic / metabolism
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Neovascularization, Physiologic / drug effects
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Receptors, Retinoic Acid / antagonists & inhibitors*
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Receptors, Retinoic Acid / metabolism
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Retinoic Acid Receptor alpha
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Transcription, Genetic / drug effects
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Tretinoin / analogs & derivatives*
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Tretinoin / pharmacology
Substances
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Antineoplastic Agents
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Klf5 protein, mouse
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Kruppel-Like Transcription Factors
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Rara protein, mouse
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Receptors, Retinoic Acid
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Retinoic Acid Receptor alpha
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Tretinoin
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3,7,11,15-tetramethyl-2,4,6,10,14-hexadecapentaenoic acid