Abstract
Hepatocyte growth factor (HGF) is a plasminogen-like protein thought to be a humoral mediator of liver regeneration. A 145-kilodalton tyrosyl phosphoprotein observed in rapid response to HGF treatment of intact target cells was identified by immunoblot analysis as the beta subunit of the c-met proto-oncogene product, a membrane-spanning tyrosine kinase. Covalent cross-linking of 125I-labeled ligand to cellular proteins of appropriate size that were recognized by antibodies to c-met directly established the c-met product as the cell-surface receptor for HGF.
MeSH terms
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Cell Line
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Cross-Linking Reagents
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Growth Substances / metabolism*
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Growth Substances / physiology
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Hepatocyte Growth Factor
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Humans
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Molecular Weight
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Phosphorylation
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Precipitin Tests
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Protein-Tyrosine Kinases / metabolism*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-met
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Receptors, Cell Surface / metabolism*
Substances
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Cross-Linking Reagents
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Growth Substances
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Receptors, Cell Surface
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Hepatocyte Growth Factor
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins c-met