Abstract
Imaging volumes as thick as whole cells at three-dimensional (3D) super-resolution is required to reveal unknown features of cellular organization. We report a light microscope that generates images with translationally invariant 30 x 30 x 75 nm resolution over a depth of several micrometers. This method, named biplane (BP) FPALM, combines a double-plane detection scheme with fluorescence photoactivation localization microscopy (FPALM) enabling 3D sub-diffraction resolution without compromising speed or sensitivity.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Biophysics / economics
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Biophysics / instrumentation
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Biophysics / methods*
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Fluorescein / pharmacology
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Fluorescent Dyes / pharmacology
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Image Enhancement
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Image Interpretation, Computer-Assisted / methods
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Imaging, Three-Dimensional / instrumentation
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Imaging, Three-Dimensional / methods*
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Lasers
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Light
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Microscopy / methods
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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Software
Substances
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Fluorescent Dyes
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Fluorescein