The ability of HDL to inhibit VCAM-1 expression and oxidized LDL uptake is impaired in renal patients

Clin Biochem. 2008 Aug;41(12):1015-8. doi: 10.1016/j.clinbiochem.2008.04.019. Epub 2008 Apr 30.

Abstract

Objectives: This study examines the ability of HDL from hemodialysis (HD) and continuous ambulatory peritoneal dialysis (CAPD) patients to suppress the expression of adhesion molecules in endothelial cells (ICAM-1, VCAM-1) and in monocytes (LFA-1, VLA-4) and to inhibit the uptake of oxidized LDL by macrophages.

Design and methods: Gene expression and the uptake of oxidized LDL were determined in 12 HD patients, 12 CAPD patients and 14 healthy volunteers.

Results: HDL from renal patients were less effective than control lipoproteins in reducing VCAM-1 expression. HDL from CAPD patients inhibited LFA-1 expression to the highest extent. The ability of HDL from renal patients to reduce oxidized LDL uptake was lower compared to control group.

Conclusions: Decreased ability of HDL to suppress expression of VCAM-1 in endothelial cells and the uptake of oxidized LDL by macrophages can be one of the risk factors for atherosclerosis development in patients with renal failure.

MeSH terms

  • Adult
  • Aged
  • Analysis of Variance
  • CD11a Antigen / metabolism
  • Cholesterol, HDL / metabolism*
  • Endothelial Cells / metabolism
  • Female
  • Humans
  • Integrin alpha4 / metabolism
  • Intercellular Adhesion Molecule-1 / metabolism
  • Kidney Failure, Chronic / metabolism*
  • Kidney Failure, Chronic / therapy
  • Lipoproteins, LDL / metabolism*
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Macrophages / metabolism
  • Male
  • Middle Aged
  • Peritoneal Dialysis, Continuous Ambulatory
  • RNA, Messenger / metabolism
  • Renal Dialysis
  • U937 Cells
  • Vascular Cell Adhesion Molecule-1 / metabolism*

Substances

  • CD11a Antigen
  • Cholesterol, HDL
  • Lipoproteins, LDL
  • Lymphocyte Function-Associated Antigen-1
  • RNA, Messenger
  • Vascular Cell Adhesion Molecule-1
  • oxidized low density lipoprotein
  • Intercellular Adhesion Molecule-1
  • Integrin alpha4