Abstract
The nucleosome assembly protein Nap1 has been implicated in various cellular functions such as histone shuttling into the nucleus, nucleosome assembly, chromatin remodelling, transcriptional control and cell-cycle regulation in Saccharomyces cerevisiae. In Schizosaccharomyces pombe nap1 null mutant cells are viable but they showed a delay in the onset of mitosis which is rescued by the absence of the replication Cds1 checkpoint kinase. In contrast, the absence of the DNA-damage Chk1 checkpoint kinase is unable to rescue the delay. Moreover, the double nap1 cds1 mutant cells lose viability and cells show positive H2AX phosphorylation, suggesting that the viability of nap1-deleted cells is due to the Cds1 kinase. We also show that overexpression of Nap1 protein blocks the cell cycle in G1 phase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / metabolism*
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Cell Proliferation
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Cell Survival
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Checkpoint Kinase 2
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Chromatin / metabolism
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Cytoskeleton / metabolism
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DNA Replication
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G1 Phase
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Gene Deletion
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Genomic Instability
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Mitosis
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Molecular Chaperones / genetics
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Molecular Chaperones / metabolism*
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Molecular Sequence Data
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Schizosaccharomyces / cytology
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Schizosaccharomyces / genetics*
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Schizosaccharomyces / metabolism
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Schizosaccharomyces pombe Proteins / genetics
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Schizosaccharomyces pombe Proteins / metabolism*
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Sequence Homology, Amino Acid
Substances
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Cell Cycle Proteins
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Chromatin
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Molecular Chaperones
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Nap1 protein, S pombe
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Nap2 protein, S pombe
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Nuclear Proteins
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Schizosaccharomyces pombe Proteins
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Checkpoint Kinase 2
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Cds1 protein, S pombe
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Protein Serine-Threonine Kinases