Constitutive expression of IL-12R beta 2 on human multiple myeloma cells delineates a novel therapeutic target

Blood. 2008 Aug 1;112(3):750-9. doi: 10.1182/blood-2008-02-139378. Epub 2008 May 12.

Abstract

The interleukin-12 (IL-12) receptor (R) B2 gene acts as tumor suppressor in human acute and chronic B-cell leukemias/lymphomas and IL-12rb2-deficient mice develop spontaneously localized plasmacytomas. With this background, we investigated the role of IL-12R beta 2 in multiple myeloma (MM) pathogenesis. Here we show the following: (1) IL-12R beta 2 was expressed in primary MM cells but down-regulated compared with normal polyclonal plasmablastic cells and plasma cells (PCs). IL-6 dampened IL-12R beta 2 expression on polyclonal plasmablastic cells and MM cells. (2) IL-12 reduced the proangiogenic activity of primary MM cells in vitro and decreased significantly (P = .001) the tumorigenicity of the NCI-H929 cell line in SCID/NOD mice by inhibiting cell proliferation and angiogenesis. The latter phenomenon was found to depend on abolished expression of a wide panel of proangiogenic genes and up-regulated expression of the antiangiogenic genes IFN-gamma, IFN-alpha, platelet factor-4, and TIMP-2. Inhibition of the angiogenic potential of primary MM cells was related to down-regulated expression of the proangiogenic genes CCL11, vascular endothelial-cadherin, CD13, and AKT and to up-regulation of an IFN-gamma-related antiangiogenic pathway. Thus, IL-12R beta 2 directly restrains MM cell growth, and targeting of IL-12 to tumor cells holds promise as new therapeutic strategy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Animals
  • Cell Proliferation / drug effects
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Interleukin-12 / pharmacology
  • Mice
  • Mice, SCID
  • Middle Aged
  • Multiple Myeloma / chemistry*
  • Multiple Myeloma / drug therapy
  • Multiple Myeloma / pathology
  • Neovascularization, Pathologic / drug therapy
  • Neovascularization, Pathologic / genetics
  • Receptors, Interleukin-12 / analysis*
  • Receptors, Interleukin-12 / genetics
  • Tumor Cells, Cultured
  • Xenograft Model Antitumor Assays

Substances

  • IL12RB2 protein, human
  • Receptors, Interleukin-12
  • Interleukin-12