Identification of G protein-coupled endothelin receptors in cultured bovine endothelial cells

S Eguchi; M Kozuka; S Hirose; T Ito; Y Hirata; F Marumo; H Hagiwara

doi:10.1016/0006-291x(91)91570-3

Identification of G protein-coupled endothelin receptors in cultured bovine endothelial cells

Biochem Biophys Res Commun. 1991 Feb 14;174(3):1343-6. doi: 10.1016/0006-291x(91)91570-3.

Authors

S Eguchi¹, M Kozuka, S Hirose, T Ito, Y Hirata, F Marumo, H Hagiwara

Affiliation

¹ Department of Biological Sciences, Tokyo Institute of Technology, Japan.

PMID: 1847632
DOI: 10.1016/0006-291x(91)91570-3

Abstract

Autocrine and paracrine regulation of vascular endothelial cells by endothelins has been postulated and has been the target of many recent investigations. In the present study, we demonstrated, by affinity labeling, the presence of endothelin-1- and endothelin-3-specific receptors on cultured bovine endothelial cells that secrete endothelin; the endothelin-3 receptor was the major form. Analysis using the GTP analogue guanosine 5'-O- (thiotriphosphate) indicated that these receptors are coupled to G proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carotid Arteries
Cattle
Cell Membrane / metabolism
Cells, Cultured
Endothelins / metabolism*
Endothelium, Vascular / metabolism*
GTP-Binding Proteins / metabolism*
Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
Kinetics
Receptors, Cell Surface / drug effects
Receptors, Cell Surface / metabolism*
Receptors, Endothelin

Substances

Endothelins
Receptors, Cell Surface
Receptors, Endothelin
Guanosine 5'-O-(3-Thiotriphosphate)
GTP-Binding Proteins