Objective: To analyze the alterations of serum protein fingerprint in patients with hypertensive disorder complicating pregnancy (HDCP), screen serum biomarker and establish a diagnostic model of HDCP.
Methods: Surface-enhanced laser desorption lionization-time of flight-mass spectrometry (SELDI-TOF-MS) technology was used to analyze serum including 25 cases of HDCP patients and 30 cases of age-, gravity- and parity-matched healthy term pregnant women on IMAC3-Cu proteinchip before delivery. Biomarker Wizard and Biomarker Pattern software was used to detect protein peaks significantly different between HDCP and controls, and establish a primary diagnostic model of HDCP. This model was further evaluated by blind test using other 16 parts of serum protein fingerprint.
Result: Ten protein peaks were significantly different at the molecular range of 2000-50000 (P < 0.01). A diagnostic model consisting of 5 protein peaks(39 837,6196,15 529, 43 248 and 22 292) was established with 100% (25/25) sensitivity, 90% (27/30) specificity, 89% (25/28) positive predictive value and 100% (27/27) negative predictive value. Blind test generated a sensitivity of 75% and specificity of 75% respectively.
Conclusion: Many differential expressed proteins exist in serum of patients with HDCP. The diagnostic model consisting of 5 protein peaks has relative high sensitivity and specificity, and can discriminate HDCP from healthy pregnant