Human respiratory syncytial virus genomic and antigenic variants isolated in two hospitals during one epidemic, in Santiago, Chile

J Clin Virol. 2008 Jul;42(3):260-3. doi: 10.1016/j.jcv.2008.03.024. Epub 2008 May 15.

Abstract

Background: Human respiratory syncytial virus (HRSV) is a major cause of severe lower respiratory tract infection (LRI) in children. Distinct variants of the viruses have been described.

Objective: The objective was to compare the antigenic and genetic variability of HRSV strains recovered from infants admitted to two hospitals during one epidemic in a big city.

Study design: We analyzed nasopharyngeal aspirates from 201 infants admitted for LRI to two hospitals during 2002 in Santiago, Chile. The analyses were carried out using a panel of monoclonal antibodies against G glycoprotein epitopes (EIA) and RFLP for N and G genes.

Results: No differences in HRSV groups A/B and in N patterns distribution were observed among both hospitals. On the contrary, antigenic and genetic G patterns displayed a wide diversity of strains circulating during one epidemic, in one big city.

Conclusions: RSV variability assessment depended rather on the tool used for analysis than on the geographical location.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral / immunology
  • Chile / epidemiology
  • DNA Fingerprinting
  • Disease Outbreaks*
  • Hospitals
  • Humans
  • Infant
  • Nasopharynx / virology
  • Polymorphism, Restriction Fragment Length
  • RNA, Viral / genetics
  • Respiratory Syncytial Virus Infections / epidemiology*
  • Respiratory Syncytial Virus Infections / virology*
  • Respiratory Syncytial Virus, Human / classification*
  • Respiratory Syncytial Virus, Human / genetics
  • Respiratory Syncytial Virus, Human / immunology
  • Respiratory Syncytial Virus, Human / isolation & purification*
  • Serotyping

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Antigens, Viral
  • RNA, Viral