Abstract
An efficient two-step recombination method for markerless gene deletion and insertion that can be used for repetitive genetic modification in Yersinia pestis was developed. The method combines lambda Red recombination and counterselective screening (sacB gene) and can be used for genetic modification of Y. pestis to construct live attenuated vaccines.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
MeSH terms
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Bacterial Proteins / genetics
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Chromosomes, Bacterial / genetics*
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Gene Deletion*
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Gene Expression Regulation, Bacterial
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Genetic Engineering / methods*
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Genetic Markers / genetics
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Mutagenesis, Insertional
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Recombination, Genetic*
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Yersinia pestis / genetics*
Substances
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Bacterial Proteins
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Genetic Markers