Abstract
The chemokine interleukin 8/CXCL8 induces the phosphorylation of the GluR1 subunit of the AMPA-type glutamate receptor in neurons and transfected HEK cells, on both serine 845 (S845) and 831 (S831) residues. We previously described that CXCL8 receptor CXCR2 and GluR1 co-precipitate and that GluR1/CXCR2 co-expression both in HEK cells and neurons impairs CXCL8-induced cell migration. Here we show that replacement of S845 with Ala (A), but not with Glu (E), strongly reduces GluR1/CXCR2 interaction and abolishes the impairment of CXCL8-induced cell migration. Considered together our findings point to the phosphorylated state of S845GluR1 as a determinant of GluR1-CXCR2 physical coupling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Newborn
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Calcium / metabolism
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Carbazoles / pharmacology
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Cells, Cultured
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Cerebellum / cytology
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Chelating Agents / pharmacology
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Chemotaxis / drug effects
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Cyclic AMP / metabolism
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Egtazic Acid / analogs & derivatives
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Egtazic Acid / pharmacology
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Enzyme Inhibitors / pharmacology
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Gene Expression Regulation / drug effects
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Gene Expression Regulation / genetics
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Hippocampus / cytology
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Humans
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Interleukin-8 / pharmacology
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Interleukin-8 / physiology*
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Mutation
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Neurons / drug effects
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Neurons / physiology
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Phosphorylation / drug effects
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Pyrroles / pharmacology
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Rats
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Rats, Sprague-Dawley
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Receptors, AMPA / drug effects*
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Receptors, AMPA / genetics
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Receptors, AMPA / metabolism*
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Time Factors
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Transfection
Substances
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Carbazoles
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Chelating Agents
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Enzyme Inhibitors
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Interleukin-8
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Pyrroles
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Receptors, AMPA
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1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
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Egtazic Acid
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KT 5720
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Cyclic AMP
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Calcium
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glutamate receptor ionotropic, AMPA 1