Functional computed tomography imaging of tumor-induced angiogenesis: preliminary results of new tracer kinetic modeling using a computer discretization approach

Radiat Med. 2008 May;26(4):213-21. doi: 10.1007/s11604-007-0217-4. Epub 2008 May 29.

Abstract

Purpose: The aim of this study was to establish functional computed tomography (CT) imaging as a method for assessing tumor-induced angiogenesis.

Materials and methods: Functional CT imaging was mathematically analyzed for 14 renal cell carcinomas by means of two-compartment modeling using a computer-discretization approach. The model incorporated diffusible kinetics of contrast medium including leakage from the capillary to the extravascular compartment and back-flux to the capillary compartment. The correlations between functional CT parameters [relative blood volume (rbv), permeability 1 (Pm1), and permeability 2 (Pm2)] and histopathological markers of angiogenesis [microvessel density (MVD) and vascular endothelial growth factor (VEGF)] were statistically analyzed.

Results: The modeling was successfully performed, showing similarity between the mathematically simulated curve and the measured time-density curve. There were significant linear correlations between MVD grade and Pm1 (r = 0.841, P = 0.001) and between VEGF grade and Pm2 (r = 0.804, P = 0.005) by Pearson's correlation coefficient.

Conclusion: This method may be a useful tool for the assessment of tumor-induced angiogenesis.

MeSH terms

  • Adult
  • Aged
  • Angiography, Digital Subtraction
  • Carcinoma, Renal Cell / diagnostic imaging*
  • Computer Simulation
  • Contrast Media / pharmacokinetics
  • Female
  • Humans
  • Immunohistochemistry
  • Iohexol / pharmacokinetics
  • Kidney Neoplasms / diagnostic imaging*
  • Male
  • Microcirculation
  • Middle Aged
  • Neovascularization, Pathologic / diagnostic imaging*
  • Prospective Studies
  • Tomography, X-Ray Computed / methods*
  • Vascular Endothelial Growth Factor A / metabolism

Substances

  • Contrast Media
  • Vascular Endothelial Growth Factor A
  • Iohexol