Abstract
Beta1 integrins play a controversial role during chondrogenesis. Since the maturation of chondrocytes relies on a signaling switch from cell-cell to cell-matrix interactions, we hypothesized that beta1 integrins play a different role at the earlier (mainly cell-cell interaction) from the later stage (mainly cell-matrix interaction) of chondrogenesis. Our data showed: in plain medium, sox9, collagen X, and collagen II gene expressions of ASCs were induced by beta1-integrin blockage at day 14. In chondrogenic medium, however, sox 9, sox6, and collagen II gene expression were induced at day 4 but inhibited at day 14. In addition, both beta1-integrin blockage and TGF-beta1 down-regulated Rock-1 and -2 gene expression and produced the round cells. We concluded that beta1 integrins play a more important role at the later stages than earlier stages of chondrogenesis, and that the onset of chondrogenesis promoted by beta1-integrin blockage might be through inhibiting Rock signaling.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / cytology*
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Adipose Tissue / drug effects
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Cell Shape / genetics
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Chondrogenesis* / genetics
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Collagen Type II / genetics
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Collagen Type X / genetics
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Culture Media
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DNA-Binding Proteins / genetics
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Gene Expression / drug effects
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Gene Expression Regulation, Developmental*
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High Mobility Group Proteins / genetics
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Humans
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Immunoglobulin G / pharmacology
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Integrin beta1 / pharmacology
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Integrin beta1 / physiology*
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SOX9 Transcription Factor
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SOXD Transcription Factors
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Stem Cells / cytology*
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Stem Cells / drug effects
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Transcription Factors / genetics
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Transforming Growth Factor beta1 / antagonists & inhibitors
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Transforming Growth Factor beta1 / pharmacology
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Transforming Growth Factor beta1 / physiology
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rho-Associated Kinases / genetics*
Substances
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Collagen Type II
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Collagen Type X
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Culture Media
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DNA-Binding Proteins
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High Mobility Group Proteins
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Immunoglobulin G
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Integrin beta1
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SOX6 protein, human
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SOX9 Transcription Factor
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SOX9 protein, human
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SOXD Transcription Factors
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Transcription Factors
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Transforming Growth Factor beta1
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rho-Associated Kinases