Abstract
The transient receptor potential canonical (TRPC) channels are Ca2+-permeable, nonselective cation channels with different biological functions, but their roles in brain are largely unknown. Here we report that TRPC6 was localized to excitatory synapses and promoted their formation via a CaMKIV-CREB-dependent pathway. TRPC6 transgenic mice showed enhancement in spine formation, and spatial learning and memory in Morris water maze. These results reveal a previously unknown role of TRPC6 in synaptic and behavioral plasticity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Calcium-Calmodulin-Dependent Protein Kinase Type 4 / genetics
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Calcium-Calmodulin-Dependent Protein Kinase Type 4 / metabolism
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Cells, Cultured
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Embryo, Mammalian
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Excitatory Postsynaptic Potentials / drug effects
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Excitatory Postsynaptic Potentials / genetics
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Excitatory Postsynaptic Potentials / physiology*
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Excitatory Postsynaptic Potentials / radiation effects
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Gene Expression Regulation, Developmental / physiology
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Hippocampus / cytology
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Maze Learning / physiology
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Mice
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Mice, Inbred C57BL
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Mice, Transgenic
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Microscopy, Confocal / methods
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Microscopy, Immunoelectron / methods
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Nerve Tissue Proteins / metabolism
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Neurons / physiology*
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Patch-Clamp Techniques / methods
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Rats
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Rats, Sprague-Dawley
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Synapses / physiology*
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Synapses / ultrastructure
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Synaptosomes / metabolism
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Synaptosomes / ultrastructure
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TRPC Cation Channels / genetics
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TRPC Cation Channels / physiology*
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TRPC6 Cation Channel
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Transfection / methods
Substances
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Nerve Tissue Proteins
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TRPC Cation Channels
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TRPC6 Cation Channel
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Trpc6 protein, mouse
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Calcium-Calmodulin-Dependent Protein Kinase Type 4
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Camk4 protein, mouse