Abstract
Quantitative mapping of the normal tissue dynamics of an entire developing mammalian organ has not been achieved so far but is essential to understand developmental processes and to provide quantitative data for computational modeling. We developed a four-dimensional (4D) imaging technique that can be used to quantitatively image tissue movements and dynamic GFP expression domains in a growing transgenic mouse limb by time-lapse optical projection tomography (OPT).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Body Patterning
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Extremities / embryology*
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Gene Expression Regulation, Developmental
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Imaging, Three-Dimensional / methods*
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In Vitro Techniques
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Mice
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Mice, Transgenic
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Time Factors
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Tomography, Optical / methods*
Substances
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Recombinant Proteins
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Green Fluorescent Proteins