Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1

Cell. 1991 May 17;65(4):551-68. doi: 10.1016/0092-8674(91)90088-g.

Abstract

We describe the cDNA encoding the SV40 transcriptional enhancer factor 1 (TEF-1) and show that its translation initiates exclusively at an AUU codon in vivo. Cloned TEF-1, which is unrelated to other known transcription factors, specifically binds the SV40 GT-IIC and Sph enhansons. Cloned TEF-1 does not activate these enhansons in lymphoid MPC11 cells where they are known to be inactive, but represses the endogenous HeLa TEF-1 activity in vivo and in vitro. Repression is also observed with chimeras where the DNA-binding domain of the GAL4 activator replaces that of TEF-1, showing that repression results from interference/squelching. Such chimeras stimulate transcription in HeLa, but not in MPC11, cells in vivo and in HeLa cell extracts in vitro. However, high concentrations result in self-interference/squelching. These results strongly suggest that the trans-activation function of TEF-1 is mediated by a highly limiting, possible cell-specific, titratable transcriptional intermediary factor(s).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular / methods
  • Codon / genetics
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / isolation & purification
  • DNA-Binding Proteins / metabolism
  • HeLa Cells / physiology
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Nuclear Proteins*
  • Oligonucleotide Probes
  • Plasmids
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Simian virus 40 / genetics
  • TEA Domain Transcription Factors
  • Transcription Factors / genetics*
  • Transcription, Genetic*
  • Transfection
  • Zinc Fingers / genetics
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Codon
  • DNA-Binding Proteins
  • Nuclear Proteins
  • Oligonucleotide Probes
  • RNA, Messenger
  • Recombinant Proteins
  • TEA Domain Transcription Factors
  • TEAD1 protein, human
  • Transcription Factors
  • beta-Galactosidase

Associated data

  • GENBANK/M60958
  • GENBANK/M60959
  • GENBANK/M60960
  • GENBANK/M60961
  • GENBANK/M60962
  • GENBANK/M63033
  • GENBANK/M63034
  • GENBANK/M63896
  • GENBANK/M64383
  • GENBANK/M64384