Most of the current methods to capture stem cells are very complicated. Our new discovery of acquiring adult stem cells by implanting three dimension (3-D) porous material into the spatium intermusculare of mice hind limbs would bring hope to achieve autologous stem cells transplantation. We discovered that a great number of cells migrated into the 3-D porous material implanted in vivo. Furthermore, the migrating cells exhibited stem cell properties (CD34(+), Sca-1(+), GFAP(+), alphafetoprotein(+)) and were hematogeous (CD45(+)) and CD105(+). The ability of migrating cells to undergo differentiation into hematopoietic lineages was tested with methylcellulose medium. These findings demonstrate that the cells captured from spatium intermusculare by implanting 3-D porous material exhibit the characteristics of stem cells.