Supercritical extraction of carotenoids from Rosa canina L. hips and their formulation with beta-cyclodextrin

AAPS PharmSciTech. 2008;9(2):693-700. doi: 10.1208/s12249-008-9100-6. Epub 2008 Jun 5.

Abstract

The purpose of this research was to preliminary assess the suitability of a new method for the preparation of a solid formulation in form of powder composed by beta-cyclodextrin and the supercritical extract of Rosa canina hips. The method implies the extraction of carotenoids, in particular beta-carotene, from freeze dried fruits of R. canina with supercritical CO2 at 70 degrees C and 300 bar, in the presence of varying quantity of ethanol as entrainer. The obtained supercritical solution is then expanded at ambient conditions into an aqueous solution of beta-cyclodextrin to favour the interaction between beta-cyclodextrin and the lipophilic components of the extract. beta-carotene solubility (mole fraction) in supercritical CO2 or in supercritical CO2/ethanol mixtures were in the order of 1 10(-7). The beta-carotene extracted from R. canina fruits (nearly 10 microg/g of dry matrix), interacts almost quantitatively with beta-cyclodextrin affording a solid phase, which presents a low apparent solubility in water. Finally the interaction with beta-cyclodextrin results in a higher concentration of the beta-carotene trans- form relative to the cis- form in the extracted product when collected in an aqueous solution of beta-cyclodextrin with respect to the extract in n-hexane.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Dioxide / chemistry
  • Carotenoids / chemistry*
  • Carotenoids / isolation & purification
  • Chemistry, Pharmaceutical
  • Chromatography, Supercritical Fluid*
  • Drug Carriers*
  • Ethanol / chemistry
  • Fruit
  • Hexanes / chemistry
  • Models, Chemical
  • Pilot Projects
  • Powders
  • Rosa* / chemistry
  • Solubility
  • Technology, Pharmaceutical / methods*
  • beta-Cyclodextrins / chemistry*

Substances

  • Drug Carriers
  • Hexanes
  • Powders
  • beta-Cyclodextrins
  • Carbon Dioxide
  • Carotenoids
  • Ethanol