Continuous synthesis of glyceryl ferulate using immobilized Candida antarctica lipase

J Oleo Sci. 2008;57(7):375-80. doi: 10.5650/jos.57.375.

Abstract

Glyceryl ferulate was synthesized through condensation of ferulic acid and glycerol at 50 degrees C in glycerols with different water contents using an immobilized lipase from Candida antarctica in a batch reactor, and condensation in the glycerol with a 7.5% (w/w) water content was shown to be the favorite. The solubility of ferulic acid was higher at higher temperature in glycerol with a lower water content. The viscosity was lower at higher temperature for the glycerol with a higher water content. The condensation was carried out using a batch reactor at a temperature from 50 degrees C to 90 degrees C. These observations indicated that the condensation at 80 degrees C in the glycerol with a 7.5% (w/w) water content was the most adequate for continuously synthesizing glyceryl ferulate. A reactor system was constructed for the continuous synthesis and was steadily operated to realize a productivity of 430 kg/(m(3)-reactor(day) without any decrease in the conversion for at least 6 days.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioreactors
  • Candida / enzymology*
  • Enzymes, Immobilized / metabolism*
  • Glycerol
  • Lipase / metabolism*
  • Monoglycerides / metabolism*
  • Solubility
  • Temperature
  • Viscosity
  • Water / metabolism

Substances

  • Enzymes, Immobilized
  • Monoglycerides
  • glyceryl ferulate
  • Water
  • Lipase
  • Glycerol